Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + MINK1 Kinase Enzyme System
Recombinant human MINK1 (1-320) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes.
Promega ERK1 Kinase Enzyme System, 10microg
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega P450-Glo™ CYP450 Assay Systems
Provide a complete set of reagents for performing luminescent cytochrome P450 assays
Promega MARK1 Kinase Enzyme System, 1mg
Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction.
Promega HIPK3 Kinase Enzyme System, 1mg
Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis.
Promega CK1 epsilon Kinase Enzyme System, 1mg
Full-length recombinant human CK1 epsilon was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1 epsilon is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes.
Promega CDK2/CyclinE1 Kinase Enzyme System, 1mg
Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
Promega BRK Kinase Enzyme System, 1mg
Recombinant full-length human BRK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. BRK is a member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domain as well as the catalytic domain.
Promega CDK9/CyclinK Kinase Enzyme System, 1mg
Recombinant full-length human CDK9 and CyclinK proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family.
Promega ADP-Glo™ Kinase Assay + ERK1 Kinase Enzyme System
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega CLK1 Kinase Enzyme System, 1mg
Recombinant human CLK1 (129-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CLK1, also known as STY, is a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases.
Promega PIP2:3PS Lipid Kinase Substrate, 0.25mg, 0.25ml
The lipid kinase substrates PIP2:3PS and PI:3PS are standalone substrates that are also part of the PI3K-Glo kinase profiling system.
Promega CDK6/CyclinD3 Kinase Enzyme System, 10microg
Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Promega PLK1 Kinase Enzyme System, 1mg
Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. A member of the Polo-Like Kinase family, PLK1 localizes to centrosomes and undergoes dramatic subcellular relocation during the cell cycle.
Promega ADP-Glo™ Kinase Assay + ACK Kinase Enzyme System
ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs.